Ben's Update 2.6.08 - 15.6.08
Dialysis of ECP for MTT
- To remove traces of tin and other impurities in the EPC
- Tin are cytotoxic and will interfer with MTT results
Procedure
1) Cut the dialysis membrane from the roll and seal its top and bottom accordingly.
2) Soak the membrane in DI water for 1hour
3) Dissolve 10% EPC in DMSO (about 0.2g)
4) Fill the container with DI water, magnetic stirrer and transfer the he EPC solution into membrane.
5) Allow it to stir inside the fridge for a week, changing the DI water each day.
6) Freeze dry to obtain EPC after a week
Paclitaxol Degradation
Taxol will go through hydrolytic degradation while doing drug release due to the strength weakness of the ester bond. This degradation results in 2 or more seperate molecules of taxol and its degradation product which will produce various peaks on the LC chromatogram. Hence, calibration for standards only applicable for undegraded taxol.
Problems to ponder, how do we quantify the degraded taxol? :(
- To remove traces of tin and other impurities in the EPC
- Tin are cytotoxic and will interfer with MTT results
Procedure
1) Cut the dialysis membrane from the roll and seal its top and bottom accordingly.
2) Soak the membrane in DI water for 1hour
3) Dissolve 10% EPC in DMSO (about 0.2g)
4) Fill the container with DI water, magnetic stirrer and transfer the he EPC solution into membrane.
5) Allow it to stir inside the fridge for a week, changing the DI water each day.
6) Freeze dry to obtain EPC after a week
Paclitaxol Degradation
Taxol will go through hydrolytic degradation while doing drug release due to the strength weakness of the ester bond. This degradation results in 2 or more seperate molecules of taxol and its degradation product which will produce various peaks on the LC chromatogram. Hence, calibration for standards only applicable for undegraded taxol.
Problems to ponder, how do we quantify the degraded taxol? :(
posted by Dr Charles Chia at
7:45 AM
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