Ben Updates 29/7/2008
1)
Experiment: BSA drug delivery EPC 6&7
Start date: 14.7
Surface area: 2.27cm2 and 2.5cm2
Concentration: 200ug & 600ug
Gel volume/vial: 0.25ml
PBS buffer volume/vial: 5ml
Total: 24vials
EPC 6 -ED i/c
EPC 7 - WH i/c
Samples of 1ml are drawn at 1h, 2.5h, 4h, 5.5h, 24h, 48h and so on till today
BSA content in sample are obtain using a MicroBCA assay kit protocol
Release curve results are available at letskillcancercells.fyp@gmail.com text me for password.
Edited by Edmund
Current sample run timing:
1h, 2.5h, 4h, 5.5h, 24h, 48h, 77h, 99h, 123h, 173h, 197h, 222h, 246h, 270h, 318h, 342h, 366h
2)
Experiment: taxol drug delivery EPC 6&7
Start date: 14/7
Surface area: smallest
Concentration: 400ug & 600ug
Gel volume/vial: 0.25ml
PBS buffer volume/vial: 6ml
Total vials: 6
Samples of 1ml are drawn at 2h, 4h, 6h, 24h, 48h and so on till today
Taxol content extracted with DCM and using 50:50 water/acn solvent to run HPLC - to be done on 30/7
Experiment: BSA drug delivery EPC 6&7
Start date: 14.7
Surface area: 2.27cm2 and 2.5cm2
Concentration: 200ug & 600ug
Gel volume/vial: 0.25ml
PBS buffer volume/vial: 5ml
Total: 24vials
EPC 6 -ED i/c
EPC 7 - WH i/c
Samples of 1ml are drawn at 1h, 2.5h, 4h, 5.5h, 24h, 48h and so on till today
BSA content in sample are obtain using a MicroBCA assay kit protocol
Release curve results are available at letskillcancercells.fyp@gmail.com text me for password.
Edited by Edmund
Current sample run timing:
1h, 2.5h, 4h, 5.5h, 24h, 48h, 77h, 99h, 123h, 173h, 197h, 222h, 246h, 270h, 318h, 342h, 366h
2)
Experiment: taxol drug delivery EPC 6&7
Start date: 14/7
Surface area: smallest
Concentration: 400ug & 600ug
Gel volume/vial: 0.25ml
PBS buffer volume/vial: 6ml
Total vials: 6
Samples of 1ml are drawn at 2h, 4h, 6h, 24h, 48h and so on till today
Taxol content extracted with DCM and using 50:50 water/acn solvent to run HPLC - to be done on 30/7
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